MagSi-NGSPREP, is designed for an optimized purification of next generation library preparation through efficient clean up of the successive enzymatic reactions (ie. End repair, dA-Tailing, Adaptor ligation). MagSi-NGSPREP is used for clean up of library targeting sequencers such as Life Technologies (ABI SOLiD, Ion Torrent), Illumina’s (HiSeq 2000, MiSeq, GAIIx), and Roche’s 454). Magnetic bead based MagSi-NGSPREP offers an efficient solution for clean-up and size selection in library preparation of NGS sequencing reactions. The simple and flexible protocol can be adjusted to your specific application and NGS platform. MagSi-NGSPREP can be used manually but is also easy to automate for high-throughput processing. In conclusion: a simple and quick way to consistent NGS results. Our partner MagnaMedics manufactures all paramagnetic beads and kits in house, starting from basic raw materials and excellent knowledge of the chemistries around.
As a consequence:MagnaMedics has full control over the bead characteristics and -specifications to meet your application needs MagnaMedics can ensure high quality products and, at the same time, affordable and very competitive pricing
The NGSPrep can be used with many platforms from many producers for instance also with the new platform from Primadiag ASCIA Line
Technical Information: Size-selection with MagSi-NGSPREP:
The size of fragments eluted from the beads (or that bind in the first place) is determined by the end concentration of agSi-NGSPREP, and this in turn is determined by the mix of DNA and beads. A 50 µl DNA sample plus 75 µl of beads will give a MagSi-NGSPREP:DNA ratio of 1.5. As this ratio is changed, the length of fragments binding and/or left in solution also changes, the lower the ratio of MagSi-NGSPREP:DNA the larger the final fragments will be at elution. Smaller fragments are retained in the buffer so you can get different size ranges from a single sample with multiple purifications. Part of the reason for this effect is that DNA fragment size affects the total charge per molecule with larger DNAs having larger charges, this promotes their electrostatic interaction with the beads and displaces smaller DNA fragments.
MagSi-NGSPREP meets the requirements to be used in the following NGS sample preparation kits:
Instrument: Illumina HiSeq / MiSeq / GAIIxIllumina TruSeq PCR-Free DNA Sample PrepIllumina TruSeq Nano DNA Sample PrepIllumina Nextera DNA Sample PrepIllumina NexteraXT DNA Sample PrepNew England Biolabs NEBNext® Ultra™ DNA Library Prep Kit for IlluminaNew England Biolabs NEBNext® DNA Library Prep Reagent Set for IlluminaNew England Biolabs NEBNext® ChIP-Seq Library Prep Reagent Set for IlluminaQIAGEN GeneRead DNA Library I Core KitKAPA Biosystems KAPA Library Preparation Kits with Standard PCR Library Amplification/Illumina series (10 libraries)KAPA Biosystems KAPA Library Preparation Kits with Real-Time PCR Library Amplification/Illumina series (10 libraries)KAPA Biosystems KAPA Low Throughput Library Preparation Kit with SPRI solution and Standard PCR Library Amplification/Illumina series (10 libraries)KAPA Biosystems KAPA Low Throughput Library Preparation Kit with SPRI solution and no PCR Library Amplification/Illumina series (10 libraries)KAPA Biosystems KAPA Library Preparation Kits with no PCR Library Amplification/Illumina series (10 libraries)Lucigen NxSeq™ DNA Sample Prep Kit 2 (Illumina Compatible)IntegenX PrepX™ ILM DNA Library ProtocolIntegenX PrepX™ ILM 32i DNA LibraryElim Biopharmaceuticals RightOnTM DNA Library Prep Kit, Illumina, v1Elim Biopharmaceuticals RightOnTM DNA Library Prep Kit, Illumina, v1, Low Input
Instrument: Life Technologies 5500 SOLiD:Life Technologies 5500 SOLiD Mate-Paired Library kitLife Technologies 5500 SOLiD Fragment Library Core kitNew England Biolabs NEBNext® DNA Library Prep Set for SOLiD
Instrument: Life Technologies Ion Proton / Ion PGM Ion OneTouch:Life Technologies Ion AmpliSeq Library Kit 2.0Life Technologies Ion Plus Fragment Library KitLife Technologies Ion Express Plus Fragment Library KitLife Technologies NEBNext® Fast DNA Library Prep Set 4New England Biolabs NEBNext® Fast DNA Library Prep Set for Ion Torrent™Life Technologies NEBNext® Fast DNA Fragmentation & Library Prep Set 4New England Biolabs NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™QIAGEN GeneRead DNA Library L Core KitNuGEN Encore® NGS Library System for Ion Torrent™NuGEN Encore NGS Multiplex Library System I for Ion Torrent NuGEN Encore NGS Multiplex Library System IB for Ion TorrentKAPA Biosystems KAPA Library Preparation Kits with Standard PCR Library Amplification/Ion Torrent series (8 libraries)KAPA Biosystems KAPA Library Preparation Kits with no Library Amplification/Ion Torrent series (8 libraries)IntegenX PrepX PGM DNA Library KitIntegenX PrepX ILM 32i DNA Library KitSwift Biosciences Accel-NGS DNA Library Kit for Ion Torrent - 10 reactions
Instrument: Roches 454 / GS FLEXNew England Biolabs NEBNext® Quick DNA Library Prep Reagent Set for 454Lucigen NxSeq™ DNA Sample Prep Kit 1 (Roche 454 Compatible)IntegenX PrepX 454 DNA Library Kit
General Information about NGSPREP:
- MagSi-NGS PREP provides a convenient tool for ultra-fast and efficient purification and size selection of DNA products. DNA fragments will be bound directly onto the surface of the magnetic beads, leaving unincorporated nucleotides, primers, primer dimers, and other contaminants in solution. Finally, the DNA fragments are eluted with low salt buffer or reagent grade water. The technology for binding of DNA fragments onto the applied magnetic nanoparticle surface does not equire use of any hazardous chaotropic buffers. The purification protocols are optimized to provide high yield and purity of the recovered DNA fragments. MagSi-NGSPREP allows selective binding of DNA with a size cut-off between 100bp and 1kb with specific reagent volume:sample volume ratio's. By increasing the volume of MagSi-NGSPREP, the efficiency of binding smaller fragments increases. This enables the user to selectively keep or discard undesired fragment sizes. Depending on which protocol is used, the total preparation time is 20-30 minutes and the hands-on time necessary for the whole procedure is reduced to a minimum. The kit can be used manually and on automated workstations using single tubes, or 96- and 384-well PCR plates. The kit is stable for 1 year when stored at 2-8°C. When working with chemicals, always wear a suitable lab coat, disposable gloves and protetective goggles. For more information, please consult the appropiate material safety sheets (MSDS). These are available.