- Medium 199 wurde ursprünglich für die Kultivierung von Hühnerembryo-Fibroblasten entwickelt. Dieses Medium verwendet Earle's Salze und hält den pH-Wert in einem CO2-Inkubator aufrecht.
- Media 199 was originally designed for the culturing of chick embryo fibroblasts. This media uses Earle's salts and will maintain pH in a CO2 incubator.
- Lagerung: 2-8°C
- Versandtemperatur: Umgebungstemperatur
- Haltbarkeit: 12 Monate
- steril filtriert
- HS: 3821 0000
- MCL-033-500ML
Bestandteile (Aminosäure mg/L):
- Glycine 50.00
- L-Alanine 25.00
- L-Arginine, Hydrochloride 70.00
- L-Aspartic Acid 30.00
- L-Cysteine, Hydrochloride, Monohydrate 0.11
- L-Cystine, Dihydrochloride 26.00
- L-Glutamine 100.00
- L-Histidine, Hydrochloride, Monohydrate 21.88
- L-Hydroxyproline 10.00
- L-Isoleucine 40.00
- L-Leucine 60.00
- L-Lysine, Hydrochloride 70.00
- L-Methionine 15.00
- L-Phenylalanine 25.00
- L-Proline 40.00
- L-Serine 25.00
- L-Threonine 30.00
- L-Tryptophan 10.00
- L-Tyrosine, Disodium, Dihydrate 57.87
- L-Valine25.00
Bestandteile (Vitamine mg/l):
- (+/-) a-Tocopherol Phosphate, Disodium Salt 0.01
- Biotin 0.01
- Choline Chloride 0.50
- D-Calcium Pantothenate 0.01
- D-Ribose 0.50
- Ergocalciferol 0.10
- Folic Acid 0.01
- L-Ascorbic Acid 0.05
- Menadione Sodium Bisulfite 0.02
- Myo-Inositol 0.05
- Niacinamide 0.03
- Nicotinic Acid 0.03
- para-Aminobenzoic Acid 0.05
- Pyridoxine, Hydrochloride 0.05
- Riboflavin 0.01
- Thiamine, Hydrochloride 0.01
- Thymine 0.30
- Uracil 0.30
- Vitamin A, Acetate 0.14
Sonstige Verbindungen mg/l:
- 2-Deoxy-D-Ribose 0.50
- Adenine Sulfate, Dihydrate 12.13
- Adenosine 5'-Monophosphate, Monohydrate 0.20
- Adenosine 5'-Triphosphate, Anhydrous 1.00
- Cholesterol 0.20
- D-Glucose, Anhydrous 1000.00
- Glutathione, Reduced 0.05
- Guanine HCl 0.30
- Hypoxanthine, Sodium 0.35
- Phenol Red, Sodium Salt 20.00
- Polysorbate 80 20.00
- Sodium Acetate, Anhydrous 50.0
Anorganische Salze mg/L
- Calcium Chloride, Anhydrous 200.00
- Ferric Nitrate, Nonahydrate 0.72
- Magnesium Sulfate, Anhydrous 97.70
- Potassium Chloride 400.00
- Sodium Bicarbonate 2200.00
- Sodium Chloride 6800.00
- Sodium Phosphate, Monobasic, Monohydrate 140.00
- Xanthine, Monosodium Salt 0.34
Spezifikation.
- Appearance Clear, red solution
- Aerobic bacteria Not detected
- Anaerobic bacteria Not detected
- Fungi (Yeast & Mold) Not detected
- pH at RT 6.9 -7.5
- Osmolality 260 -340 mM/kg
- Endotoxin? 1.0 EU/ml
- Mycoplasma Not detected
- Cell Culture -Cell Growth Promotion 3 Passages
- Viability ? 75
