The microvascular endothelial cell growth medium is a sterile liquid culture medium for culturing human microvascular endothelial cells (HMVEC). The medium is delivered as a basal medium and is suitable for culturing HMVEC after adding the optional available essential supplement kit components. The final formulation is optimized for initial seeding of 4,000 cells / cm² up to confluence (approx. 90 %). Feeder-layer, matrix substrates or other substances are not necessary.
The supplemented microvascular endothelial cell growth medium can be stored in the dark at 4°C to 8°C for up to 1 month. Do not heat the medium over 37°C or use uncontrollable sources of heat (e.g. microwave appliances). If only a part of the medium is to be used, remove this amount from the bottle and heat it.
Do not freeze the medium. This can lead to high salt concentrations by freezing out pure water which will cause irreversible damage
The microvascular endothelial cell growth medium is thoroughly tested after each production. All components are tested in a stringent biological assay. Each batch is checked for HMVEC proliferating characteristics. The cells cultured in microvascular endothelial cell growth medium are checked regarding their morphology, the adherence rate, the colony forming efficiency and the population doubling time
The pH is set at 7.6 and osmolality at 285 ± 10 mOsm / kg.
In vitro laboratory use only: Not intended for any human or animal diagnostic or therapeutic use