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Art.Nr.
MagSi-gDNA blood kit 96 for fast and economic automated extraction of genomic DNA
Laborbedarf, Molekularbiologie,Magnetic Beads,Blood Kit

  • from a wide range of sample volumes
  • The kit is suitable for use with whole blood which has been prepared with commonly used anticoagulants like EDTA, citrate or heparin. The reagent volumes can be scaled up or down to be used for different sample volumes, making it possible to use the products in any situation where gDNA isolation out of blood is needed.

    Features:

  • Suitable for whole blood, prepared with citrate, EDTA or heparin
  • Reproducible, high yield of DNA – up to 8 ?g from 200 ?L fresh or frozen whole blood
  • Excellent purity, A260/280 = 1.7-2.0
  • Suitable for many genomic applications including PCR, DNA sequencing and southern blotting
  • Simplify your routine:

  • Simple bind-wash-elute procedure
  • Protocol easily adjustable for different sample volumes
  • Manual and automated use, also with Primadiag pipetting platform
  • Safe sample handling with minimal user input
  • Easy to automate:

    • Compatible with many different automated liquid handling systems (e.g Thermo ScientificTM KingFisherTM, PrimaDiag PrimaRWS®, Gilson pipetmaX®, Eppendorf epMotion®, Tecan Freedom EVO®, Hamilton MICROLAB® STAR Line/NIMBUS, PerkinElmer JANUS®)
    • Fast and robust protocols
    • Suitable for small, medium and high-throughput automation
    • Large volume sample input possible

    The MagSi-gDNA blood kit allow purification of genomic DNA of fresh or frozen whole blood samples using magnetic beads and any liquid handling workstation. The scalabillity of our MagSi-gDNA blood kits makes it possible to use the products in any situation where gDNA isolation out of blood is needed from single experiments using the MM-Separators leading though to high throughput platforms of which the KingFisher® is a perfect and widely used example.

    Advantages:

  • Ultra pure, ready to use genomic DNA for enhanced performance in sensitive applications
  • Erythrocyte lysis is not necessary
  • High binding capacity
  • Reproducible yields from large blood volumes
  • No cross contaminations
  • Standardized processing ensures reproducible results
  • Optimal preservation of gDNA
  • Low amounts of (chemical) waste
  • Ordering of single different kit components possible
  • In combination with the liquid handling systems the automated MagSi gDNA blood kits enables purification of DNA from 1 - 96 samples of whole human blood or other commonly used blood components (buffy coat, serum). After filling the standard containers (single tubes, 96 deepwell plates, all required isolation steps are robottically performed. In contrast to other blood kits available using blood volumes > 1 ml, lysis of erythrocytes is not required. Blood sample lysed with the MagSi gDNA blood kit is optimal as the lysis buffer contains Proteinase K. In combination with the robotic workstation the lysis step can be carried out direct on the platform at ambient temperature or via the integrated thermomixer available for most robots at 56°C. Further processing is done by making use of the magnetic capabillities of the MagSi DNA beads. The free DNA binds to magnetic particles, followed by washing steps and the final elution. The high quality DNA can be stored at “20 °C or is ready to use for subsequent downstream applications like:

  • PCR, Real-time PCR
  • RFLP analysis
  • Restriction enzyme digestion
  • Hybridisation
  • SNP analysis
  • Genotyping
  • Magnetic bead-based kit is a fast and economic purification system for extraction of genomic DNA from mammalian samples. The MagSi-gDNA blood kit includes magnetic beads, buffers and Proteinase K, and is easy to automate for high-throughput processing.


     
     

    BESTELLUNG

    Menge

     
    Pack
      Volume
      96 preps 180.00 EUR   MD60001 W
     10x96 preps 1350.00 EUR   MD61001 W
     10x960 preps   MD62001 W

    URL zu diesem Artikel: http://www.baacklab.de/shop/de/?artnr=MD60001 W

     

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