from a wide range of sample volumes
The kit is suitable for use with whole blood which has been prepared with commonly used anticoagulants like EDTA, citrate or heparin. The reagent volumes can be scaled up or down to be used for different sample volumes, making it possible to use the products in any situation where gDNA isolation out of blood is needed.
Features:Suitable for whole blood, prepared with citrate, EDTA or heparinReproducible, high yield of DNA – up to 8 ?g from 200 ?L fresh or frozen whole bloodExcellent purity, A260/280 = 1.7-2.0Suitable for many genomic applications including PCR, DNA sequencing and southern blotting
Simplify your routine:Simple bind-wash-elute procedureProtocol easily adjustable for different sample volumesManual and automated use, also with Primadiag pipetting platformSafe sample handling with minimal user input
Easy to automate:
- Compatible with many different automated liquid handling systems (e.g Thermo ScientificTM KingFisherTM, PrimaDiag PrimaRWS®, Gilson pipetmaX®, Eppendorf epMotion®, Tecan Freedom EVO®, Hamilton MICROLAB® STAR Line/NIMBUS, PerkinElmer JANUS®)
- Fast and robust protocols
- Suitable for small, medium and high-throughput automation
- Large volume sample input possible
The MagSi-gDNA blood kit allow purification of genomic DNA of fresh or frozen whole blood samples using magnetic beads and any liquid handling workstation. The scalabillity of our MagSi-gDNA blood kits makes it possible to use the products in any situation where gDNA isolation out of blood is needed from single experiments using the MM-Separators leading though to high throughput platforms of which the KingFisherÂ® is a perfect and widely used example.
Advantages:Ultra pure, ready to use genomic DNA for enhanced performance in sensitive applicationsErythrocyte lysis is not necessaryHigh binding capacityReproducible yields from large blood volumesNo cross contaminationsStandardized processing ensures reproducible resultsOptimal preservation of gDNALow amounts of (chemical) wasteOrdering of single different kit components possible
In combination with the liquid handling systems the automated MagSi gDNA blood kits enables purification of DNA from 1 - 96 samples of whole human blood or other commonly used blood components (buffy coat, serum). After filling the standard containers (single tubes, 96 deepwell plates, all required isolation steps are robottically performed. In contrast to other blood kits available using blood volumes > 1 ml, lysis of erythrocytes is not required. Blood sample lysed with the MagSi gDNA blood kit is optimal as the lysis buffer contains Proteinase K. In combination with the robotic workstation the lysis step can be carried out direct on the platform at ambient temperature or via the integrated thermomixer available for most robots at 56°C. Further processing is done by making use of the magnetic capabillities of the MagSi DNA beads. The free DNA binds to magnetic particles, followed by washing steps and the final elution. The high quality DNA can be stored at “20 °C or is ready to use for subsequent downstream applications like:PCR, Real-time PCRRFLP analysisRestriction enzyme digestionHybridisationSNP analysisGenotyping
Magnetic bead-based kit is a fast and economic purification system for extraction of genomic DNA from mammalian samples. The MagSi-gDNA blood kit includes magnetic beads, buffers and Proteinase K, and is easy to automate for high-throughput processing.